Skip to main content
. Author manuscript; available in PMC: 2021 Jun 14.
Published in final edited form as: Oncogene. 2021 May 12;40(23):3929–3941. doi: 10.1038/s41388-021-01798-2

Figure 7. Induced ERK5 silencing suppresses breast cancer lung metastasis.

Figure 7

Control (dox-) and doxycycline-fed (dox+) mice bearing mammary tumor grafts were paired according to similar tumor size. (A, B) Breast metastasis was detected post mortem by quantification of live (DAPI-) breast cancer cells (tdT+) in the lung. A representative flow cytometry analysis is shown in (B). The graphical analysis of the data demonstrated that ERK5 knockdown significantly reduced metastatic burden. Ratio paired t test was applied for p value calculation, p ≤ 0.01 (**). Raw numbers utilized for the graph are presented in the table. (C) Breast metastasis to the spleen and bone were detected by bioluminescence imaging in one control (dox-) animal of a pair sacrificed at 4 weeks after dox exposure.