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. Author manuscript; available in PMC: 2021 Oct 4.
Published in final edited form as: J Cell Biol. 2021 Jul 22;220(10):e202104093. doi: 10.1083/jcb.202104093

Figure 1. Increasing TCR signal strength increases CTL dwell time.

Figure 1

OTI CTL expressing Lifeact-mApple (green) and RFP-PACT (green sphere) interacting with EL4 (blue), pulsed with N4, T4, G4 or NP68 peptides (a) Representative timeseries of OTI CTLs encountering targets, numbered sequentially. Scale bars = 5μm (b) Violin plot showing dwell times for individual CTLs with targets; number of interactions N4 n=121, T4 n=128, G4 n=169, NP68 n=294; bars represent median with quartiles. A Bonferroni corrected Mann-Whitney test was used for statistical analysis with *=p<0.5 and ****=p<0.0001. (c) Data from (b) was used to calculate the mean number of interactions per CTL per time-series, and plot the mean per independent movie (N4 n=30, T4 n=34, G4 n=30, NP68 n=42). Bars represent median with quartiles.