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. Author manuscript; available in PMC: 2021 Oct 4.
Published in final edited form as: J Cell Biol. 2021 Jul 22;220(10):e202104093. doi: 10.1083/jcb.202104093

Figure 5. Calcium flux precedes centrosome polarisation and uropod retraction.

Figure 5

OTI CTL expressing GCAMP6m (green), Lifeact-mApple (red) and RFP-PACT (red sphere) interacting with EL4 (blue), pulsed with N4. (a-b) Representative max-intensity projection timeseries of OTI CTL encountering N4 pulsed target. Scale bars = 5μm, time min:sec post contact with target. (b-c) Example cell from (a) segmented with Imaris to measure: (b) the distance of the distal pole (blue) and centrosome (red) to the synapse. (c) The mean GCaMP6m fluorescence within the CTL. (d-g) Violin plots of (d) time from contact to first calcium flux, n=38, the time from the start of the calcium flux to; (e) start of uropod retraction (n=24), (f) start of centrosome polarisation toward synapse (n=34), (g) centrosome docking at the synapse (n=32).