Figure 3. Progeroid phenotype in LmnaHGPSrev/HGPSrev (HGPSrev) mice with ubiquitous progerin expression.
(A) Postnatal body-weight curves (n=14 WT; n=22 HGPSrev). Differences were analyzed by unpaired multiple t-tests and the Holm-Sidák correction. (B) Representative images of ≈8- and ≈13-month-old mice. Scale bar, 2 cm. (C) Representative images of hematoxylin & eosin-stained skin from ≈13-month-old mice and the results of subcutaneous fat layer (SFL) score and thickness quantification (see Methods) (n=13-14 WT; n=11-12 HGPSrev). Statistical analysis was performed by two-tailed t-test. Scale bar, 500 μm. (D) Representative images of sagittal whole-body cross-sections obtained by MRI (fat shown in white) and quantification of body-fat mass and percentage fat content in ≈13-month-old mice (n=23 WT; n=10 HGPSrev). Differences were analyzed by two-tailed t-test. (E) Kaplan-Meier survival curve (n=13 WT; n=22 HGPSrev). Differences were analyzed by the Mantel-Cox test. ***, p<0.001; ****, p<0.0001. Data are mean±SEM. Each symbol represents one animal.