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. Author manuscript; available in PMC: 2022 Apr 2.
Published in final edited form as: J Proteome Res. 2022 Mar 15;21(4):1189–1195. doi: 10.1021/acs.jproteome.1c00771

Table 1. Comparison of the supported features of ProForma 1.0 and 2.0.

Feature ProForma 1.0 ProForma 2.0
Protein modifications designated by CV/ontology names and accession numbers
Representation of glycan composition
N-terminal and C-terminal modifications
Delta mass notation for modifications
Information tag
Joint representation of experimental data and interpretation
NEW Support for elemental formulas Limited
NEW Representation of isotopes Limited
NEW Cross-link notation X
NEW Representation of inter-chain cross-links X
NEW Representation of disulfide linkages X
NEW Representation of glycans with GNO ontology as CV X
NEW Specifying a gap of known mass X
NEW Labile modifications X
NEW Unknown modification position X
NEW Possible set of modification positions X
NEW Representing ranges of positions for the modifications X
NEW Modification position preference and localization scores X
NEW Scoring for ranges of positions for a modification X
NEW Fixed protein modifications X
NEW Ambiguity in the order of amino acid sequences X
NEW Representation of ion charges and more than one peptidoform per spectrum X
NEW Representation of branched peptides X
NEW Representation of ambiguity in the order of the amino acid sequence X