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. Author manuscript; available in PMC: 2022 May 28.
Published in final edited form as: Diabetes. 2022 May 1;71(5):1115–1127. doi: 10.2337/db21-1166

Figure 1. Cre recombinase expression driven by the adiponectin promoter does not reduce Gipr mRNA transcripts in adipose tissue.

Figure 1

A) Gipr expression in epididymal white adipose tissue (WAT) and interscapular brown adipose tissue (BAT). Cre expression is driven by transgenic expression of Adipoq-Cre (36). (n=3-8). B) Gipr, Cre, and Adipoq expression in epididymal WAT and interscapular BAT. For reference, the average cycle threshold (ct) values for Gipr and Adipoq were 26.28 and 18.77, respectively, in WAT and 28.13 and 20.73, respectively, in BAT. Cre expression is driven by the AdipoqBAC promoter (AdipoqBAC-Cre; (37)). (n=3). C) Gipr expression in various adipose tissue depots in mice with the AdipoqBAC-Cre transgene. (n=3). D) Cre and Gipr expression in tissues, including epididymal WAT from various Cre recombinase models crossed with Giprflx/flx mice. (n=2-3). For relative RNA expression values, in panels A-C, the values are normalized to expression in the Cre controls (A – Adipoq-Cre, B,C – AdipoqBAC-Cre). For panel D, the values are normalized to levels for MIP-CreERT* - p<0.05 vs control.