Extended Data Fig. 4. Western Blots of eGFP and Rheb_eGFP.

The eGFP, Rheb_eGFP and Nucleolin protein levels of HEK293T and HeLa cell samples transfected with either ApppG-, m⁷GpppG- or NPM- capped mRNA were analyzed via Western blotting at 24 h post transfection. Irradiation of transfected cells was performed 4 h post transfection at 365 nm for 30 sec. As marker, the prestainedPageRuler (ThermoFisher) was used and as primary antibodies anti-nucleolin-antibody or anti-eGFP-antibody were used, respectively. Additionally, a HRP secondary antibody was used. Shown is one representative gel from n=3 independent experiments.