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. Author manuscript; available in PMC: 2022 Aug 10.
Published in final edited form as: Nat Chem. 2022 Jun 20;14(8):905–913. doi: 10.1038/s41557-022-00972-7

Figure 3. Characterization of FlashCaps.

Figure 3

a) Absorption spectra of indicated guanosines and cap analogues. b) Time- and wavelength-dependent photo-deprotection for indicated caged guanosines. c) Stability of FlashCaps in cell lysate at 37 °C in comparison to cap 0. d-e) Affinity measurements of FlashCaps before and after irradiation with eIF4E (Cy5-labelled) using microscale thermophoresis (MST). The average of three independent measurements is shown. f) Stability of FlashCaps against enzymatic degradation by DcpS with or without irradiation at 15 min (dashed line) (365 nm, 30 s). g) Stability of irradiatedFlashCaps against enzymatic degradation by DcpS (365 nm, 30 s) in comparison to cap 0. Data of n=3 independent experiments are shown as mean values +/- SD.