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. Author manuscript; available in PMC: 2022 Oct 28.
Published in final edited form as: Oncogene. 2022 Sep 28:10.1038/s41388-022-02471-y. doi: 10.1038/s41388-022-02471-y

Figure 7. HMG20B knockdown in murine hematopoietic stem and progenitor cells.

Figure 7

(A-C) Murine bone marrow KIT+ cells were infected with a lentiviral vector targeting Hmg20b or Gfi1 for KD, or a non-targeting control (NTC), and selected with puromycin for 48 hrs prior to semisolid culture. (A) KD efficiency for the indicated genes at 72 hrs, as determined by qPCR. (B) Bar chart shows mean+SEM percentage of the indicated cell types in cytospin preparations after seven days in semisolid culture (n=3). * indicates P< 0.05 for the indicated comparisons by one way ANOVA with a Tukey post hoc test. (C) Exemplar cytospin preparations. Mat, maturing; Mac, macrophage; Neut, neutrophil. (D) Exemplar flow cytometry plots. (E) Bar chart shows mean+SEM percentage of cells with the indicated immunophenotype after seven days in semisolid culture (n=2); and (F) mean+SEM ratio of immunophenotypic granulocytes (Ly6Ghi F4/80lo) to macrophages (Ly6Gint F4/80hi). Control = untransduced or NTC (n=4); Hmg20b KD = KD #1 and #2 (n=4); Gfi1 KD (n=2). * indicates P< 0.05 for the indicated comparisons by one way ANOVA with a Tukey post hoc test.