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. Author manuscript; available in PMC: 2022 Sep 30.
Published in final edited form as: J Neurosci. 2022 Jul 14;42(33):6453–6468. doi: 10.1523/JNEUROSCI.0521-22.2022

Figure 3. The abundance of FL-APP and CTF is not altered by trisomy of Hsa21.

Figure 3

(A-D) The relative abundance of full-length APP (FL-APP), APP β-C-terminal fragment (β-CTF) and APP α-C-terminal fragment (α-CTF) compared to β-actin was measured by western blot using A8717 primary antibody in the cortex at 3-months of age in female and male mice. (A) Significantly less FL-APP was observed in mice in which App was humanised and mutated (F(1,19) = 23.837, p < 0.001). An additional copy of Hsa21 did not alter FL-APP abundance (F(1,19) = 0.599, p = 0.449). (B) Significantly less CTF-α was observed in mice in which App was humanised and mutated (F(1,19) = 5.950, p = 0.025) but an additional copy of Hsa21 did not alter α-CTF abundance (F(1,19) = 3.012, p = 0.099). (C) Significantly more β-CTF was observed in mice in which App was humanised and mutated (F(1,19) = 868.431, p < 0.001). By ANOVA a significant effect of Hsa21 on CTF-β abundance was detected (F(1,19) = 23.462, p < 0.001), however wild-type (WT) and Tc1 (Bonferroni pair-wise comparison p = 1.000) and AppNL-F/NL-F and Tc1;AppNL-F/NL-F (Bonferroni pair-wise comparison p = 0.118) were not statistically significant. WT female n = 4, male n = 4: Tc1 female n = 3, male n = 5; AppNL-F/NL-F female n = 2, male n = 2; Tc1;AppNL-F/NL-F female n = 4, male n = 4. (D) Representative image of western blot in WT, Tc1, AppNL-F/NL-F, Tc1;AppNL-F/NL-F mice. Error bars show SEM, data points are independent mice.