Skip to main content
. Author manuscript; available in PMC: 2022 Sep 30.
Published in final edited form as: J Neurosci. 2022 Jul 14;42(33):6453–6468. doi: 10.1523/JNEUROSCI.0521-22.2022

Figure 5. Biochemical solubility of amyloid-β42 is not altered by an additional copy of the Dp3Tyb, Dp(10)2Yey or Dp(17)3Yey Hsa21 orthologous regions.

Figure 5

Total cortical proteins were biochemically fractionated from 8-month of age mice and amyloid-β abundance analysed by MSD assay (6E10). No difference in the abundance of (A) 5 M guanidine hydrochloride soluble amyloid-β42 (F(1,12) = 0.128, p = 0.726), (D) 1% Triton X-100 soluble amyloid-β42 (F(1,12) = 2.863, p = 0.116) or (G) Tris-soluble amyloid-β42 (F(1,10) = 0.281, p = 0.608) was observed between Dp3Tyb; AppNL-F/NL-F compared with AppNL-F/NL-F controls. AppNL-F/NL-F female n = 2, male n = 6; Dp3Tyb;AppNL-F/NL-F female n = 7, male n = 3. No difference in the abundance of (B) 5 M guanidine hydrochloride soluble (F(1,11) = 2.337, p = 0.115), (E) 1% Triton X-100-soluble amyloid-β42 (F(1,11) = 0.145, p = 0.711) or (H) Tris-soluble amyloid-β42 (F(1,11) = 0.001, p = 0.978) was observed between Dp(10)2Yey;AppNL-F/NL-F compared with AppNL-F/NL-F controls. AppNL-F/NL-F female n = 4, male n = 5; Dp(10)2Yey;AppNL-F/NL-F female n = 6, male n = 3. No difference in the abundance of (C) 5 M guanidine hydrochloride soluble (F(1,20) = 4.079, p = 0.057), (F) 1% Triton X-100 soluble amyloid-β42 (F(1,20) = 0.124, p = 0.728) or (I) Tris soluble (F(1,20) = 0.521, p = 0.479) amyloid-β42 was observed between Dp(17)3Yey;AppNL-F/NL-F compared with AppNL-F/NL-F controls. AppNL-F/NL-F female n = 7, male n = 5; Dp(17)3Yey;AppNL-F/NL-F female n = 7, male n = 7. Details of negative controls, which do not carry an AppNL-F allele in Table 2. Dp3Tyb abbreviated to Dp3, Dp(10)2Yey abbreviated to Dp10, Dp(17)3Yey abbreviated to Dp17 for clarity. Error bars show SEM, data points are independent mice.