Total cortical proteins were homogenised in TBS buffer, from mice of 3-months of age, and amyloid-β abundance was analysed by MSD assay (4G8). An additional copy of the Dp3Tyb region did not alter the abundance of (A) amyloid-β40 (F1,14) = 0.693, p = 0.419) or (B) amyloid-β42 (F1,7) = 0.410, p = 0.718). Wild-type (WT) (female n = 5, male n = 11), Dp3Tyb (female n = 7, male n = 12). For amyloid-β40 (WT n = 4 and Dp3Tyb = 5) and amyloid-β42 (WT= 8 and Dp3Tyb n = 8) were below the limit of detection. Error bars show SEM, data points are independent mice. Dp3Tyb abbreviated to Dp3 for clarity.