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. Author manuscript; available in PMC: 2023 Jan 22.
Published in final edited form as: Nat Cell Biol. 2023 Jan 9;25(1):108–119. doi: 10.1038/s41556-022-01042-3

Extended Data Figure 4. Instability of nuclear envelope blebs in melanoma cells.

Extended Data Figure 4

(a) Percentage of nuclear envelope blebs positive for DNA damage response markers (53BP1 and γ-H2AX) in primary melanoma WM983A cells and metastatic melanoma WM983B cells. N=2. (b) Representative pictures of a WM983B nucleus stained for γ-H2AX (green), 53BP1 (magenta) and DNA (blue). Scale bars, 10 μm. (c) Relative γ-H2AX and 53BP1 fluorescence intensity levels (left) and average γ-H2AX and 53BP1 foci per nucleus (right) in WM983A and WM983B cells with or without nuclear envelope blebs. n= 246 and 283 cells, respectively. (d,e) Representative image sequence from repetitive, transient nuclear envelope rupture events in WM983A cells (d) or WM983B cells (e) stably expressing GFP-NLS and imaged over 5 hours. Images showing the first frame of a nuclear envelope rupture in (d) or (e) are surrounded by a magenta square. Scale bars, 10 μm. Numerical data are available in the Source Data.