(a) Quantification of LAP1B (left) and LAP1C (right) expression in metastatic melanoma WM983B cells after 48 hours of treatment with a siGENOME SMARTpool targeting LAP1. (b) Percentage of alive WM983B cells after a first round of transwell migration upon LAP1 depletion with a siGENOME SMARTpool. (c) Representative immunoblot for LAP1 expression levels in WM983B cells after 48 hours of LAP1 depletion with ON-TARGETplus individual siRNAs. Western blot was probed with anti-GAPDH and anti-LAP1 antisera (d) Quantification of LAP1B (left) and LAP1C (right) expression in WM983B cells after 48 hours of LAP1 depletion with ON-TARGETplus individual siRNAs as in (c). (e,f) Percentage of WM983B cells that translocated their nuclei (e) and displayed nuclear envelope blebs (f) after a second round of transwell migration through 5-μm pores upon LAP1 depletion with ON-TARGETplus individual siRNAs. n= 772, 632 and 590 cells, respectively. Bar charts show the mean and error bars represent S.E.M. from N = 3 independent experiments. P-values calculated by one-way ANOVA (d,e,f) and unpaired two-tailed t-test (a,b); *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Unprocessed western blots, numerical data and exact p-values are available in the Source Data.