(a) Resolved soluble (S) and insoluble (P) fractions from WM983B cells subject to the indicated extractions were examined by western blotting with antisera raised against LAP1, GAPDH or Lamin B1. Western blot representative of N = 2 independent experiments. (b) Schematic of the different N-terminal domains of LAP1 isoforms and cartoon illustrating the mitochondrial retargeting assay. (c) Representative images of mitochondrial retargeting assay in cells expressing mEmerald-Lamin A/C (left) or mEmerald-Lamin B1 (right) and stained for HA (magenta) and DNA (blue). Scale bars, 10 μm. (d) Percentage of cells showing co-localisation of mEmerald-Lamin A/C (left, n= 210, 211 and 190 cells, respectively) or mEmerald-Lamin B1 (right, n= 211, 199 and 183 cells, respectively) with LAP1BNT/ LAP1B1-121/LAP1CNT. Bar charts show the mean and error bars represent S.E.M. from N = 3 independent experiments. P-values calculated by one-way ANOVA (d); *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Unprocessed western blots and numerical data are available in the Source Data.