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. Author manuscript; available in PMC: 2023 Jan 21.
Published in final edited form as: JCI Insight. 2022 Dec 22;7(24):e154142. doi: 10.1172/jci.insight.154142

Figure 1. Synthesis of antibody-PMO conjugates.

Figure 1

(A) Schematic of conjugation synthesis between 25-mer PMO to the free thiol group (SH) of a solvent-exposed, engineered cysteine residue in the CH2 domain of the heavy chain of either a low-affinity mouse TfR antibody (8D3130) or an isotype control antibody (nitrophenol, NIP228). PPMO, peptide linker-phosphorodiamidate morpholino oligonucleotide. (B) Chemistry of linker between PMO and antibodies. (C) MALDI-TOF spectra of the unmodified antibodies (black trace) and the purified antibody-PMO conjugates (red traces) for the 8D3130 (left) and NIP228 antibodies (right).