Figure 1.

Hp-TGM reduces airway eosinophilia in response to Alternaria allergen. Alternaria allergen (10 μg) mixed with or without Hp-TGM (5 μg) was intranasally administered and mice culled 24 h later for recovery of bronchoalveolar lavage fluids (BALFs) and lung tissues. (a) Schematic representation of the experimental protocol. (b) Total cell numbers in BALF from control mice and those receiving Alternaria allergen (Alt) or Alternaria with Hp-TGM (Alt-TGM). (c) Representative flow cytometry plots of SiglecF vs. CD11c expression on CD45⁺ BALF cells from individual mice from the three experimental groups; percentage of eosinophils shown in inset boxes for each plot. (d) Numbers of eosinophils (CD45⁺CD11b⁺CD11c⁻Ly6C⁻Ly6G⁻SiglecF⁺) in BALF determined by flow cytometry. (d) Numbers of eosinophils (CD45⁺CD11b⁺CD11c⁻Ly6C⁻Ly6G⁻SiglecF⁺) in lung homogenates determined by flow cvtometrv. (f and g) Numbers of eosinophils (CD45⁺CD11b⁺CD11c⁻Ly6C⁻Ly6G⁻SiglecF⁺) in BALF (f) and lung homogenates (g) of RAG-1 deficient mice determined by flow cytometry. Results are pooled from two independent experiments each with 2–4 mice per group. All data were analvsed using Prism (Graphpad Software Inc.), one-wav ANOVA with Tukev’s multiple comparisons post-test was used to compare multiple independent groups. Mean values and SEM are indicated. ****p < 0.0001; **p < 0.01; *p < 0.05; non-significant differences not shown.