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. Author manuscript; available in PMC: 2023 Jan 31.
Published in final edited form as: Immunology. 2022 Jul 12;167(2):197–211. doi: 10.1111/imm.13528

Figure 4.

Figure 4

Hp-TGM administrated at challenge reduces allergic responses to house dust mite. Mice were sensitized with house dust mite allergen (3 μg) intranasally at day 0. Allergic responses to HDM were recalled daily from day 7 to day 11 (10 μg) and Hp-TGM (1 μg) was administered intraperitoneally every day of challenge. Mice were then culled at day 15. (a) Schematic representation of the experimental protocol. (b and c) Total cell (b) and eosinophil (c) numbers were enumerated in BALF. (d) Total cell numbers enumerated in lung homogenates. (e and f) Eosinophil cell numbers enumerated in lung homogenates (e) and representative flow cytometry plot of SiglecF versus CD11c expression on lung cells gated on live CD45+ cells. Percentage of eosinophils shown in inset boxes for each plot (f). Data are from ≥2 independent experiments (n = 7-9). All data were analysed using Prism (Graphpad Software Inc.), one-way ANOVA with Tukey’s multiple comparisons post-test was used to compare multiple independent groups. Mean values and SEM are indicated. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; nonsignificant differences not shown.