Figure 5.

Inhibition of eosinophilia by Hp-TGM is associated with lower IL-4 and eotaxin-1 responses. Using the same protocol as previously described (Figure 4a), type 2 cytokine and regulatory gene responses were assessed by Luminex and/or qPCR. (a–e) Lung homogenate levels of eotaxin-1, IL-4, IL-5, IL-13 and CCL17 were measured. (f and g) Expression of Il5 and Il13 transcripts. (h) Percentage of Foxp3+ within all CD4+ T cells. (i) Expression of Foxp3 transcripts in lung homogenates. Data are from ≥2 independent experiments, Data shown are pooled from two experiments (n = 7–9). All data were analysed using Prism (Graphpad Software Inc.), one-way ANOVA with Tukey’s multiple comparisons post-test was used to compare multiple independent groups. Mean values and SEM are indicated. ****p < 0.0001; **p < 0.01; *p < 0.05; non-significant differences not shown.