Figure-8. Identification of a GAS-like motif in Alu elements.
(A) Multiple sequence alignment (Muscle; EBI) of human and mouse retroelement sequences downloaded from the dfam database (n=66 sequences). The Alu-GAS motif (CCTGTAATCCCAGC) identified by STAT1 and STAT3 ChIP-seq is located. Conserved regions are shown in blue, and a summary of the sequence identity is shown in orange (0-100%). (B) An annotated summary retroelement sequence is shown (top) locating representative secondary motifs (+/-150bp) relative to the Alu-GAS motif. Spaced motif analysis (Spamo; Meme ChIP suite) of these secondary sites are shown. (C) Quantitation of retroelement binding in ChIP-seq datasets based on repeatmasker annotations.