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. Author manuscript; available in PMC: 2023 Nov 7.
Published in final edited form as: J Immunol. 2023 Nov 15;211(10):1506–1515. doi: 10.4049/jimmunol.2300318

Figure 3. GC B cells from aged donor mice have no intrinsic defects in cMyc upregulation.

Figure 3

(a) Representative flow cytometric plots showing the gating strategy for cMyc+ cells from donor GC B cells (CD45.1+ CD19+ B220+ NP+ CD38- GL7+ CD95+) and LZ (CXCR4lo CD86hi) GC B cells. (b) Flow cytometric plots showing the overlay of cMyc+ GC B cells (blue) on total GC B cells (red) gated for dark zone (CXCR4+CD86lo) and light zone (CXCR4loCD86+) phenotype from young adult or aged B1-8i donor mice. Numbers adjacent to gates indicate percentage of donor NP+ B220+ CD19+ CD38- GL7+ CD95+ GC B cells. (c-d) Graphs depicting the percentage of cMyc+ GC B cells out of donor NP+ GC B cells (c) and donor NP+ LZ GC B cells (d) in recipient iLNs at day 6 post-transfer and immunisation. Bar height corresponds to the mean, error bars indicate standard deviation, and each symbol represents values from individual recipient mice. Statistics were calculated using unpaired Mann-Whitney U test. Data pooled from three independent repeat experiments.