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. Author manuscript; available in PMC: 2023 Nov 7.
Published in final edited form as: J Immunol. 2023 Nov 15;211(10):1506–1515. doi: 10.4049/jimmunol.2300318

Table 1. List of antibodies used for flow cytometric analysis of B1-8i cells pre-transfer.

Antibodies used in Surface Stain Company and Clone Identifier Dilution
1 BUV563-coupled anti-mouse CXCR4 BD (2B11/CXCR4) 741313 1:400
2 BV480-coupled anti-mouse IgG1 BD (A85-1) 746811 1:500
3 BV785-coupled anti-mouse CXCR5 BioLegend (L138D7) 145523 1:200
4 PE-coupled NP Biosearch Technologies N-5070-1 1:100
5 PE-Cy7-coupled anti-mouse CD40 BioLegend (3/23) 124622 1:2000
6 A700-coupled anti-mouse MHCII IA/IE Invitrogen (M5/114.15.2) 56-5321-82 1:2000
7 ViaKrome808-coupled Live/Dead Beckman Coulter C36628 1:2000
8 APC-Fire750-coupled anti-mouse CD86 BioLegend (GL-1) 105045 1:1000
Antibodies used in Intracellular Stain Company and Clone Identifier Dilution
9 BUV395-coupled anti-mouse CD11c BD (N418) 744180 1:1000
10 BUV496-coupled anti-mouse CD23 BD (B3B4) 741058 1:2000
11 BUV661-coupled anti-mouse CD19 BD (1D3) 612971 1:2000
12 BUV737-coupled anti-mouse CD62L BD (MEL-14) 612833 1:1000
13 Pacific Blue-coupled IRF4 BioLegend (IRF4.3E4) 646418 1:1000
14 BV510-coupled anti-mouse CD21 BD (7G6) 747764 1:2000
15 AF488-coupled anti-mouse GL7 Invitrogen (GL-7) 53-5902-82 1:1000
16 Spark YG593-coupled anti-mouse CD11b BioLegend (M1/70) 101282 1:2000
17 Alexa Fluor 594-coupled anti-mouse Tbet BioLegend (4B10) 644833 1:500
18 PE-Fire 640-coupled anti-mouse CD38 BioLegend (90) 102744 1:2000
19 Spark NIR685-coupled anti-mouse IgD BioLegend (11-26c.2a) 405750 1:2000
20 APCFire810-coupled anti-mouse B220 BioLegend (RA3-6B2) 103278 1:1000