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. Author manuscript; available in PMC: 2023 Dec 2.
Published in final edited form as: Cereb Cortex. 2023 Oct 17;33(23):11354–11372. doi: 10.1093/cercor/bhad371

Figure 3. Functional synaptic input locally to L1 from L5 Tlx3 and Sim1 neurons in S1 cortex.

Figure 3

(A) Experimental setup to test the input from L5 Tlx3-Cre and L5 Sim1-Cre neurons onto L1 interneurons. (B) Example recordings of input to L1 interneurons from Tlx3 (top) and Sim1 (bottom) neurons. (C) Group data of evoked EPSPs in L1 interneurons (Tlx3, n=5; Sim1, n=5, three mice each genotype). (D) Short-term dynamics of evoked EPSPs in L1 interneurons(10 Hz; Tlx3, n=5; Sim1, n=5, three mice each genotype). (E, F) Top, experimental setup to test the input from L5 IT (Tlx3) and L5 PT (Sim1) neurons onto subcellular spots along L5b PT (n=8, three mice) and L5a IT (n=5, three mice) neurons, respectively. Middle, example neurons and their responses to light spot illumination in the indicated locations on each neuron (E, L5b neuron, input from Tlx3; F, L5a neuron, input from Sim1). Bottom, group data comparing the input to L1 (apical tufts) and L5 (somatic region). A-D, experiments performed in normal ACSF solution; E, F, experiments in the presence of TTX and 4-AP.