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. Author manuscript; available in PMC: 2013 May 28.
Published in final edited form as: J Neurosci. 2012 Nov 28;32(48):17086–17096. doi: 10.1523/JNEUROSCI.1391-12.2012

Figure 5. Role of hdc in other neurons which undergo remodeling.

Figure 5

A-C, We used C161-GAL4 to express UAS-CD8::GFP in 5 other da neurons and stain the wL3 fillets with HDC antibody. hdc is expressed in the other class of da neurons, in dbd neuron (purple arrows) and in ddaC (yellow arrow head).

D-F, We used C161-GAL4 expressing UAS-CD8::GFP to visualize dorsal sensory neurons (D). In wild-type pupae at 24h APF (E), only 2 da neurons and dbd neuron remain in the field (arrow), and they have undergone pruning. The 3 other da neurons labeled in C161-GAL4 have undergo apoptosis by this time. F, In hdc43/hdc43 at 24h APF, ddaA, B and F are absent, demonstrating that hdc is not required for the apoptosis of da neurons. The remaining neurons, ddaA, ddaE and dbd show consistent severing defects (arrow).

G-I, 24h APF γ-MB neurons labeled with 201Y-Gal4>UAS-mCD8-GFP. G, γ-MB neurons have almost completely pruned both their axons (arrows) and dendrites. H, In MARCM clones for hdc43, the pruning does not appear to be modified, and only a few axons remain (arrows). I, Pruning is suppressed by down-regulating EcR with EcR-RNAiCA104, resulting in the persistence of axons at 24h APF (arrows).