. Author manuscript; available in PMC: 2013 Jul 2.

Published in final edited form as: Pflugers Arch. 2013 Feb 9;465(7):997–1010. doi: 10.1007/s00424-013-1224-1

Table 1.

Composition of pipette solutions (PS) used to detect mitoplast Ca²⁺ currents in the mitoplast-attached configuration

	Composition of pipette solution (mM)	Appearance of i-MCC	Appearance of xl-MCC
PS1	105 CaCl₂	Yes	Yes
	10 HEPES
	0.01 CsA
	0.01 CGP 37187
PS2	65 Ca MeS	Yes	Yes
	40 CaCl₂
	10 HEPES
	0.01 CsA
	0.01 CGP 37187
PS3	220 sucrose	No	No
	35 NMDG-Cl
	10 HEPES
	2 EGTA
	0.1 DIDS
	0.01 CsA
	0.01 CGP 37187

The pH of all solutions was adjusted to 7.2 with KOH. In all experiments, bath solution contained (in millimolars): 150 KCl, 1 EGTA, 1 EDTA, 10 HEPES, pH was adjusted to 7.2 with KOH