Table 2.
Gating characteristics of the detected mitoplast Ca2+ currents
NPo(ms) | Tcmean | Tomean(ms) | Number | |
---|---|---|---|---|
i-MCC | 0.61±0.14 | 3.5±0.5 | 14.9±2.4 | 13 |
xl-MCC | 0.77±0.06 | 45.8±14.3 | 57.7±15.9 | 8 |
b-MCC | 0.34±0.08 | 4.4±0.9 | 22.6±2.6 | 5 |
In all experiments, bath solution contained (in mM): 150 KCl, 1 EGTA, 1 EDTA, and 10 HEPES and pH was adjusted to 7.2 with KOH. Open probability (NPo), mean open time (Tomean) and mean close time (Tcmean) of intermediate (i-MCC), extra-large mitochondrial Ca2+ channel (xl-MCC) and burst (b-MCC) mitochondrial Ca2+ channels are presented as means ± SEM. n, provides the number of individual patches for the respective experiments