Table 1. Primer and probe sequences (5′ to 3′) used in the measurement of mRNA levels by real-time polymerase chain reaction.
Target Gene | Primer/probe | Sequence | Accession no. |
---|---|---|---|
GR | Forward Primer | ACTGCCCCAAGTGAAAACAGA | X70407 |
Reverse Primer | GCCCAGTTTCTCCTGCTTAATTAC | ||
TaqMan Probe | AGAAGATTTTATCGAACTCTGCACCCCTGG | ||
| |||
G6Pase | Forward Primer | TGGAGTCTTTTCAGGCATTGC | EF062861 |
Reverse Primer | CTTGAGACTGGCATTGTAGATGCT | ||
TaqMan Probe | TTGCTGAGACTTTCCGCCACATCCA | ||
| |||
PEPCK | Forward Primer | GATTGGCATCGAGCTGACAGA | EF062862 |
Reverse Primer | CGCCCATCCTCGTCATG | ||
TaqMan Probe | TCGCCCTACGTGGTGACCAGCA | ||
| |||
InsR | Forward Primer | ACC-GCC-AAG-GGC-AAG-AC | AY162434 |
Reverse Primer | AGC-ACC-GCT-CCA-CAA-ACT-G | ||
TaqMan Probe | AAC-TGC-CCT-GCC-ACT-GTC-ATC-AAC-G |