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. Author manuscript; available in PMC: 2013 Nov 15.
Published in final edited form as: Mol Microbiol. 2013 Jun 27;89(3):10.1111/mmi.12285. doi: 10.1111/mmi.12285

Fig. 4.

Fig. 4

TbMLP down-regulation leads to enhanced susceptibilty to iron chelators. Cells were induced for 24 hours and then plated in varying concentrations of deferoxamine (DFO) or SHAM. Plates were incubated for 72 hours and growth measured using alamarBlue™. The baseline was calculated using alamarBlue™ incubated with medium alone. Control cells (2T1 strain) are shown in the left hand panels and two independent RNAi clones in the centre and right hand panels. Solid line indicates cells incubated without tetracycline (−tet) and broken line indicates cells induced with tetracycline (+tet). EC50 values (+/−SEM) are illustrated in the table. EC50 values were determined using Graphpad PRISM software and the significance of the difference between EC50 values (+/−tet) was calculated using the F-test (P-values shown in table).