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. Author manuscript; available in PMC: 2015 Apr 23.
Published in final edited form as: Oncogene. 2013 Oct 28;33(43):5100–5108. doi: 10.1038/onc.2013.447

Figure 1. BASP1 associates with prohibitin in the nucleus.

Figure 1

(A) Nuclear extracts from V-K562 (V) cells and BASP1-K562 (B) cells were used in immunoprecipitation with anti-FLAG antibodies. The immunoprecipitates were immunoblotted with anti-BASP1 antibodies. (B) FLAG immunoprecipitates from part A were resolved by electrophoresis and the gel was stained with Sypro Ruby. BASP1 and prohibitin were identified by MALDI-TOF mass spectrometry. (C) HeLa cell nuclear extracts were subjected to immunoprecipitation with control IgG or anti-prohibitin antibodies and immunoblotted with anti-BASP1 antibodies. I is input. (D) Same as in part C except that the immunoprecipitation was performed with anti-BASP1 antibodies and the samples immunoblotted with anti-prohibitin antibodies. (E) MCF7 cells were used to perform coimmunoflurescence with anti-rabbit BASP1 antibodies and anti-mouse prohibitin antibodies and visualized with confocal microscopy. Hoechst was used to label the nucleus. Immunofluorescence with control anti-rabbit (r) and anti-mouse (m) antibodies are shown in the bottom row. (F) Same as in part (E) except that U2OS cells were used.