. Author manuscript; available in PMC: 2014 May 17.

Published in final edited form as: J Vis Exp. 2014 Mar 30;(85):10.3791/51242. doi: 10.3791/51242

Table 3. Enzyme mix for the reverse transcription reaction.

Reagent	Volume (ml)/reaction	Concentration/reaction
First Strand Buffer (10x)	1	1
MgCl₂ (25 mM)	2	4
DTT (0.1 M)	1	0.008
RNaseOUT (40 U/ml )	0.5	16
Superscript III Reverse Transcriptase (200U/ml )	0.5	8
Total	5