. Author manuscript; available in PMC: 2014 May 17.

Published in final edited form as: J Vis Exp. 2014 Mar 30;(85):10.3791/51242. doi: 10.3791/51242

Table 4. Master mix for the nested PCR.

Reagent	Volume (ml)/reaction	Final Concentration/Reaction
DEPC treated water	18.4	-
PCR Buffer (10x)	2.5	1
MgCl₂ (50 mM)	1	2
dNTP mix (10 mM)	0.5	0.2
Froward primer (5 pmol/ml)	0.25	0.05
Reverse primer (5 pmol/ml)	0.25	0.05
Platinum Taq Polymerase (5 U/ml)	0.1	0.02
Subtotal	23	-