Table 5. Master mix for the sequencing reactions.
| Reagent | Volume (ml)/reaction | Concentration/reaction |
|---|---|---|
| DEPC treated water | 6.1 | |
| Sequencing Buffer (5x) | 2 | 1 |
| Primer (3.2 pmol/ml) | 0.5 | 0.16 |
| Big Dye terminator Sequencing mix | 0.4 | - |
| Total | 9 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.