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Mono-amination of diketone 3a to yield Δ1-piperideine 6a. Conditions: lyophilized E. coli cells containing the overexpressed ω-TA (20 mg), diketone 3a (12 mg, 50 mm), PLP (1 mm), NAD+ (1 mm), ammonium formate (150 mm), d- respective l-alanine (500 mm), AlaDH (12 U), FDH (11 U), organic co-solvent and KPi buffer (100 mm, pH 7.0), 30 °C, 24 hours in an Eppendorf orbital shaker (700 rpm).
