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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Immunol Cell Biol. 2014 Oct 28;93(2):167–176. doi: 10.1038/icb.2014.90

Figure 4. Ackr2-deficiency leads to an increased Th17 response during the induction of arthritis.

Figure 4

Eight days after challenge with CII, draining lymph nodes were harvested from WT littermate control (WT) and Ackr2-deficient (Ackr2−/−) DBA/1j mice (n = 5) and the recall immune response evaluated. Single cell suspensions of lymph node cells (3×105 cells/well) were stimulated with CII (60μg/ml) or medium alone and assessed for (A) specific proliferation (i.e. proliferation after CII stimulation minus proliferation in medium alone) or (B-C) the levels of IFNγ and IL-17 in the medium. **, p < 0.01 by a Kruskal-Wallis test with Dunn’s multiple comparison post-test. A repeat experiment yielded similar results.