Data in left and right panels are from two separate experiments with elongated plants (legends in A and B). Jasmonic acid (JA, A-B), jasmonoyl isoleucine (JA-Ile, C-D), salicylic acid (SA, E-F), and abscisic acid (ABA, G-H) were quantified in the first fully expanded leaf (+1) after no treatment (Con) or 1 h after treatment with W+W or W+OS, which is within the peak of elicited jasmonates in Nicotiana attenuata. A very small amount of JA and JA-Ile was detected in constitutive measurements (<0.1 μg for JA, <0.01 μg for JA-Ile). There were no significant differences (corrected P>0.05) in Welch’s t-tests or Wilcoxon rank-sum tests between lines and WT within each treatment; the Holm-Bonferroni method was used to correct for multiple testing (WT tested twice within each treatment).