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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Plant Physiol. 2014 Sep 3;166(2):779–797. doi: 10.1104/pp.114.247130

Figure 4. Expression of TPS10 and TPS10M does not alter levels of defense metabolites constitutively, or after elicitation (mean ± SEM, n = 4).

Figure 4

Data in left and right panels are from two separate experiments with elongated plants (legends above A and B). Samples were taken from the 0 leaf (source-sink transition) 3 d after treatment, to permit elicited metabolite synthesis and accumulation. Nicotine (A-B), rutin (C-D), chlorogenic acid (E-F), chryptochlorogenic acid (G-H), caffeoylputrescine (I-J), and total hydroxygeranyllinalool diterpene glycosides (HGL-DTGs, K-L) were quantified after no treatment (Con) or after treatment with W+W, W+OS, Lan, or Lan+MJ. Cryptochlorogenic acid and caffeoylputrescine were quantified as chlorogenic acid equivalents (CA equiv); nd, not detected. Peak areas of individual HGL-DTGs are shown in Tables S1 and S2. *P < 0.05 in a Welch’s t-test between line M-1 and WT in the Con treatment (rutin, D). There were no other significant differences (ns, not significant: P>0.05) after Holm-Bonferroni correction of Welch’s t-tests or Wilcoxon rank-sum tests between lines and WT within each treatment (WT tested twice versus lines 10-3 and 10-4, individual and total HGL-DTGS tested).