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. Author manuscript; available in PMC: 2014 Sep 27.
Published in final edited form as: J Neurosci. 2010 Sep 8;30(36):12138–12150. doi: 10.1523/JNEUROSCI.3245-10.2010

Figure 4. Early stress increases the prevalence of spontaneous network activity (up states) in the presence of DOI.

Figure 4

A, Examples from control (Ai) and ES (Aii) recordings in current-clamp showing the lack (control) and the occurrence (ES) of episodes of spontaneous network activity detected by layer V pyramidal neurons after application of the 5-HT2 partial agonist, DOI (3 μm; 15 min), to the brain slice. One episode is enlarged to show detail of the spontaneously occurring up state observed. These phenomena were identified based on previous description of network activity in cortical brain slices (see Materials and Methods). B, To quantify the amplitude of the network activity, voltage-clamp recording were performed. Examples of control (rare) (Bi) and ES (Bii) network activity are shown in voltage-clamp. The horizontal gray line denotes the measured amplitude of the up state.