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. Author manuscript; available in PMC: 2014 Dec 16.
Published in final edited form as: Nat Commun. 2014 Nov 27;5:5576. doi: 10.1038/ncomms6576

Figure 2. Two distinct lncRNAs are transcribed from a bidirectional promoter upstream of tgp1+.

Figure 2

(a) Previously published strand-specific RNA-Seq analysis (Rhind et al., 2011) upstream of SPBC1271.09/tgp1+, represented as RPKM (reads per kilobase per million). Location of qPCR primer pairs and probes for northern analysis are shown below. (b) Rbp1 ChIP-qPCR experiments performed in wild-type cells. (c, e, g) Northern analysis of nc-1343, nc-tgp1, and tgp1+ transcript levels in wild-type, rrp6Δ, mmi1Δ, and 1343Δ. (d, f, h) RT-qPCR experiments measured nc-1343, nc-tgp1, and tgp1+ transcript levels in wild-type, rrp6Δ, mmi1Δ and 1343Δ. Error bars represent SEM resulting from at least three independent replicates.