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. Author manuscript; available in PMC: 2014 Dec 16.
Published in final edited form as: Nat Commun. 2014 Nov 27;5:5576. doi: 10.1038/ncomms6576

Figure 5. tgp1+ is not regulated by RNAi/heterochromatin.

Figure 5

(a) H3K9me2 ChIP-qPCR experiments performed in the presence or absence of phosphate. clr4Δ was used as a negative control. The euchromatic actin gene (act1+) and centromeric dg repeats (dg) are positive and negative controls for heterochromatin. pho1+ is a phosphate-regulated gene repressed by nc-pho1, a lncRNA target of Mmi1. sme2+ is another lncRNA target of Mmi1. H3K9me2 to bulk H3 ratio has not been presented due to background methyl H3K9 levels detected at these loci. (b) RT-qPCR experiments measured tgp1+, nc-tgp1, and nc-1343 transcript levels in wild-type cells and cells lacking factors involved in heterochromatin formation and stability. Error bars represent SEM resulting from at least three independent replicates.