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. Author manuscript; available in PMC: 2015 Jul 16.
Published in final edited form as: Nat Commun. 2015 Jan 16;6:6043. doi: 10.1038/ncomms7043

Figure 3. Jas9-VENUS can be used to study long-distance JA signalling in planta.

Figure 3

(a) Seedlings were wounded by cutting a cotyledon using dissection scissors. Time course quantification of Jas9-VENUS fluorescence, normalised to H2B-RFP fluorescence, in the inner and outer tissues of the root over 120 minutes after wounding. Error bars show the SEM of 13 replicates. (b) Time course quantification of Jas9-VENUS fluorescence, normalised to H2B-RFP fluorescence, in the inner tissues at four positions along the root (indicated on the diagram) before and after wounding. (c) Shoots and roots of 1-week-old WT or aos seedlings were harvested 30 minutes and 2 hours after wounding and the expression of wound-inducible genes were investigated using RT qPCR. The experiment was repeated twice and the results show a representative dataset. Error bars represent the SD of four technical replicates.