Figure 5. Effects of Indole on L Cells in Primary Murine Colonic Cultures.
(A) Representative trace showing the GCaMP3 fluorescence, reporting the intracellular Ca2+ level, in a primary colonic L cell perfused with 1 mM indole and 30 mM KCl, as indicated by the horizontal bars.
(B) Mean GCaMP3 fluorescence from L cells, recorded as in (A), before and during indole perfusion, after indole washout, and during perfusion with 30 mM KCl. Values are means ± SEM for nine cells.
(C) GLP-1 release measured from primary colonic cultures incubated for 2 hr in the presence of 10 mM glucose, with and without 1 mM indole, as indicated. Each data point is calculated by averaging over six independent measurements.
*p < 0.05, **p < 0.01 by Student’s t test.