Skip to main content
. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Am J Respir Crit Care Med. 2015 Jan 1;191(1):54–62. doi: 10.1164/rccm.201402-0314OC

Figure 1. Comparison of GR expression at baseline and effect of dexamethasone on GR expression.

Figure 1

(A) Baseline GR expression in ASMC of healthy subjects and asthma patients. After serum starvation for 24 hours, GR and β-actin protein in whole cell lysates from ASMC of healthy subjects and non-severe and severe asthma patients were measured in duplicate along with a standard (STD) protein by Western Blot followed by densitometric analysis. Representative blots are shown. Further analysis shows the comparison of baseline GR expression between the non-severe and severe asthma patients. Horizontal lines represent median. Kruskal-Wallis, post hoc test: **p<0.01, *p<0.05. (B) Effect of dexamethasone on GR expression. ASMC were treated with dexamethasone (10−7 M) for times indicated. GR and β-actin protein in the whole cell lysates were measured by Western Blot followed by densitometric analysis. Bars represent mean ± SEM from 3 ASMC of healthy subjects. ** p<0.01 vs unstimulated (US).