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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Am J Respir Crit Care Med. 2015 Jan 1;191(1):54–62. doi: 10.1164/rccm.201402-0314OC

Figure 2. Impaired nuclear translocation of GR in ASMC of severe asthma.

Figure 2

(A) Nuclear GR expression at baseline. After serum starvation for 24 hours, GR and TBP protein in nuclear extracts from ASMC of healthy subjects and non-severe and severe asthma patients were measured in duplicate along with a standard (STD) protein by Western Blot followed by densitometric analysis; representative blot is shown. Horizontal lines represent median. (B) Dexamethasone-induced nuclear translocation of GR. ASMC of healthy subjects (●, n=8) and non-severe (■, n=8) and severe asthma (▲, n=8) patients were treated with dexamethasone (10−7 M) for times indicated, GR and TATA-box binding protein (TBP) in the nuclear extracts, as well as a standard (STD) protein, was assessed by Western blot followed by densitometric analysis. Points represent mean ± SEM. * p<0.05 vs healthy subjects. # p<0.05, ## p<0.01 vs non-severe asthma.