. Author manuscript; available in PMC: 2015 Apr 7.

Published in final edited form as: Extremophiles. 2015 Jan 21;19(2):451–467. doi: 10.1007/s00792-015-0730-9

Table 2.

Alanine mutations of selected amino acids of Agl3: enzymatic characterization of wild-type Agl3 and the mutants E147A and Y148A

Enzyme protein	Total reaction volume (μl)	Amount of enzyme (mg)	Total yield (Units^a)	Specific activity (U mg⁻¹ of protein)	Total activity (%)
Wild-type Agl3	200	0.121	0.00673	0.0556	100
E147A mutant	200	0.135	0.00094	0.0069	12.4
Y148A	200	0.129	0.00109	0.0084	15

One unit is defined as micromole of product (UDP-d-glucose-5,6-ene) produced per milligrams of enzyme protein in 30 min