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. Author manuscript; available in PMC: 2015 Jun 18.
Published in final edited form as: Nat Commun. 2015 May 28;6:7203. doi: 10.1038/ncomms8203

Figure 4. Induction of RUNX1-ETO in committed myeloid progenitor cells.

Figure 4

(a) Outline of myeloid progenitor culture and induction strategy in progenitor expansion medium. (b) Percentage and number of up- and down- regulated genes in myeloid progenitor cultures after overnight RUNX1-ETO induction compared to all expressed genes. The numbers over the bars represent the number of genes changing expression. (c) PCA demonstrating the differential effect of RUNX1-ETO induction on gene expression in 4 different cell types induced at different stages (HE, HE2, CD41+KIT+Tie2− progenitors, myeloid progenitors). RUNX1-ETO induced gene expression changes in myeloid progenitors is markedly different from that in early progenitors where RUNX1-ETO was induced in the HE stage. (d) Heatmap demonstrating differential gene expression change of direct RUNX1-ETO targets in the different cell types after RUNX1-ETO induction. (e) Venn diagram demonstrating that there is little overlap between RUNX1-ETO target genes changing expression in the different cell types.