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. Author manuscript; available in PMC: 2015 Jul 21.
Published in final edited form as: Nat Commun. 2015 Jul 7;6:7499. doi: 10.1038/ncomms8499

Figure1. RNF111 is necessary for efficient GG-NER.

Figure1

(A) Representative pictures of Unscheduled DNA Synthesis of the indicated MEFs, determined by EdU incorporation over 3 h after UV-irradiation (16 J m−2). Scale bar, 25 μm. (B) Quantification of UDS levels in MEFs, as determined by EdU incorporation over a time period of 3 h or 9 h after UV-irradiation (16 J m−2). UDS levels in WT MEFs were set at 100% (n>100 cells per sample, in at least two independent experiment; error bars are the mean ± SD). (C) 6-4PP removal assayed by immunofluorescence, using a 6-4PP specific antibody. The indicated MEFs were UV-irradiated (10 J m−2) and allowed to repair 6-4PPs for the indicated time points. Relative fluorescence directly after UV was set at 100%. (n>70 cells, three independent experiments; error bars are the mean ± SD).