Skip to main content
. Author manuscript; available in PMC: 2015 Jul 21.
Published in final edited form as: J Immunol. 2015 Jun 19;195(3):882–891. doi: 10.4049/jimmunol.1403110

Figure 3. Mtb-driven collagen degradation is MT1-MMP dependent.

Figure 3

A-B. Human monocytes were seeded on FITC conjugated Type I Collagen and infected with Mtb. Collagen degradation was analysed by immunofluorescent microscopy. Blue: DAPI nuclear stain, Green: Type I Collagen. Mtb infection causes greater collagen degradation. C. MT1-MMP activity was neutralised with an inhibitory anti-MT1-MMP antibody (MAB3328, Millipore) at 10μg/ml. MT1-MMP inhibition inhibited areas of collagen breakdown. D. An isotype control antibody did not inhibit collagen degradation. E. Fluorescence quantitation confirms collagen degradation is MT1-MMP-dependent. Data are from a single experiment and are representative of 3 independent experiments. Mean values +/− SD is shown, ***p<0.001 by One-way ANOVA with Tukey’s post hoc test.