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. Author manuscript; available in PMC: 2015 Jul 21.
Published in final edited form as: J Immunol. 2015 Jun 19;195(3):882–891. doi: 10.4049/jimmunol.1403110

Figure 4. MT1-MMP in expressed in TB granulomas from patients and is driven by monocyte-monocyte networks.

Figure 4

A. Lung biopsies from 5 patients with culture proven Mtb infection and control tissue from uninvolved lung parenchyma of patients with lung cancer were immunostained for MT1-MMP. In control biopsies, MT1-MMP immunostaining is present only in alveolar macrophages (i and ii). In TB, MT1-MMP immunoreactivity is present throughout the granuloma, expressed by both giant cells and epithelioid macrophages (iii and iv). White arrows indicate Langerhans multinucleate giant cells, surrounded by epithelioid macrophages. B. Monocytes were stimulated with Conditioned Medium from Mtb infected monocytes (CoMTb) to model intercellular networks. MT1-MMP expression was measured in unpermeabalised and permeabalised monocytes by immunofluorescent staining and microscopy. Blue: DAPI nuclear stain, Magenta: MT1-MMP. CoMTb stimulation increased both surface and intracellular MT1-MMP expression at 24h. Images are representative of 3 independent experiments.