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. Author manuscript; available in PMC: 2016 Aug 3.
Published in final edited form as: J Proteomics. 2015 Jun 3;126:172–188. doi: 10.1016/j.jprot.2015.05.030

Figure 11. O-glycan analyses by NSI-MS and LC-MS.

Figure 11

O-glycans from Aedes (A), Anopheles (B) and Drosophila (C) larvae were released by reductive β-elimination, permethylated, enriched in the organic (DCM) phase and analysed by NSI-MS in positive ion mode. Singly charged mainly core-1 modified O-glycans were detected. Glucuronylated O-glycans (m/z 752, 998, 1216) were predominantly found in Drosophila larvae. The automated TIM MS2 spectra of the three glucuronylated structures found in Anopheles (D, F) and in Drosophila (E, G) larvae are presenting a mixture of similar daughter ions. Fragmentation schemes are shown in each MS2 spectra. Native, β-eliminated O-glycans from Aedes (H) and Anopheles (I) were also analysed by LC-MS in negative ion mode.