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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Nat Cell Biol. 2015 Aug 3;17(9):1182–1192. doi: 10.1038/ncb3214

Figure 3. ISC tumor initiation and outgrowth requires autonomous Spi/EGFR signaling.

Figure 3

(a-c) Phosphorylated histone H3 Ser10 (red) and GFP (green) after expression of NRNAi (a), EGFRRNAi and NRNAi (b) and spiRNAi and NRNAi (c) with esgts for 3 days.

(d) Phosphorylated histone H3 Ser10 (red) and GFP (green) after expression of spiRNAi and NRNAi with esgts for 14 days.

(e) Mean number of phosphorylated histone H3 Ser10 positive cells per midgut with s.e.m. after expression with esgts of NRNAi and GFP (n=30 midguts), NRNAi and EgfrRNAi (n=30 midguts, Mann-Whitney: p=0.0001), NRNAi and spiRNAi for 3 (n=33 midguts, Mann-Whitney: p<0.0001) or 30 days, and NRNAi and Mkp3 for 5 or 10 days. Midguts were pooled from 3 independent experiments.

(f) Survival after expression of GFP alone (control), spiRNAi, NRNAi, and NRNAi and spiRNAi with esgts (n=100 flies per genotype pooled from 2 independent experiments).

DNA is a-d (blue). Scale bars in a-c, 40 μm; d, 50μm.